Chinese Virus unusual sequence in a specific location

 THE BEGINNING – Unknown Virus

 

April 2012 six workers in Mojiang mine fell ill from a mystery illness while removing bat feces. Three of the six subsequently died.

 

From first becoming too sick to work in the mine, patient 2 survived 57 days until he died. Patient 3 survived 120 days after stopping work. Patient 4 survived 117 days and then was discharged as cured. Each had been exposed in the mine for 14 days prior to the onset of severe symptoms and they were treated for infections with symptoms (on admission) of:  a) dry cough, b) sputum, c) high fevers, especially shortly before death, 

d) difficulty breathing, and e) myalgia (sore limbs). Four had low blood oxygen diagnosed as ARDS (Acute Respiratory Distress Syndrome) and immune damage considered indicative of viral infection. Tendency for thrombosis was noted in patients 2 and 4.  Symptom severity and mortality were age-related (though from a sample of 6 this must be considered anecdotal). Potential common and rare causes of their symptoms were tested for and eliminated. Treatment included ventilation (patients 2-4), steroids (all patients), antivirals (all except patient 5), and blood thinners (patients 2 and 4).   

Remote meetings were held with Zhong Nanshan at Sun Yat-Sen University, Guangdong. Zhong is the Chinese hero of the SARS epidemic, a virologist, and arguably the most famous scientist in China. Samples from the miners were later sent to the Wuhan Lab and to Zhong Nanshan. The source of infection was concluded to be  a horseshoe bat with unknown coronavirus, not SARS virus. Samples were taken from patients for “scientific research” and blood samples (at least) were sent to the Wuhan Lab testing positive for serum IgM suggesting the existence of virus infection.  June 27th, 2012, the doctors performed an unexplained thymectomy on patient 4. The thymus is an immune organ that can potentially be removed without greatly harming the patient and it could have contained large quantities of virus. 

Science magazine wrote up part of the incident in 2014 as A New Killer Virus in China?

 

MIDDLE – Virus named BtCoV/4991 (and covertly renamed RaTG13)

Virol Sin. 2016 Feb; 31(1): 31–40.Coexistence of multiple coronaviruses in several bat colonies in an abandoned mineshaft

BtCoV/4991, a partial sequence of coronavirus and RaTG13, a full sequence of a coronavirus, are from the same bat fecal sample from Mojiang mine in 2013.

 

Wuahn lab has great interest in SARS-like bat coronaviruses, searched the Mojiang mine for bat viruses on four separate occasions between August 2012 and July 2013, even though the mine is a 1,000 Km from Wuhan, collecting trips began while some of the miners were still hospitalized.

 

Partial sequence by Wuhan lab first described in 2016, BtCoV/4991 was a 370 nucleotide virus fragment collected from the Mojiang mine in 2013 by the Wuhan Lab.

 

In 2017 and 2018 Wuhan lab working with BtCoV/4991 with full sequencing.   Full sequence has one segment 100% identical to BtCoV/4991

 

In February 2020, Wuhan Lab published full sequence of BtCoV/1991 but gave it a new name, RaTG13, without mentioning BtCov/1991 when they had cited BtCoV/4991 twice in earlier publications and once in a genome sequence database .  Further there was no mention of miners or deaths RaTG13 

 

END – SARS Cov2 Pandemics hits the world
 

RaTG13 coronavirus is the closest corona virus to SARS Cov2.

MEANING  - Coronavirus RaTG13 (aka BtCoV/1991) was the likely virus that infected 9 minors from Mojjian mine in 2012. RaTG13 likely mutated or was modified into the more infectious and dangerous SARS Cov2 under selective pressure within an human/laboratory environment  over prolonged period of time/study. Wuhan Lab had SARS Cov2 in various samples, including thymus, taken from Miners in 2012. Given Wuhan Lab’s extensive work with corona viruses it is likely that SARS Cov2 was accidentally released from the Wuhan Lab. 

On Sept. 12, 2019, coro­n­avirus bat se­quences were deleted from the in­sti­tute’s data­base. Why? It changed the se­cu­rity pro­to­cols for the lab. Why? It put out re­quests for more than $600 mil­lion for a new ven­ti­la­tion sys­tem. What prompted this new need?

The story of SARS-Cov-2 started long be­fore Jan­uary 2020. We be­lieve the virus was most likely un­con­tained in a lab­o­ra­tory where it was be­ing worked on, and that it es­caped un­in­ten­tion­ally. A Har­vard study of satel­lite im­ages re­vealed a shut­down of traf­fic around the Wuhan lab in the late sum­mer and early fall of 2019. Weeks later, in late Sep­tember, the hos­pi­tal park­ing lots were fill­ing up.

Unusual Sequence in Precise Location 

Sars Cov 2 has a very unusual sequence,  ccgccg, in a very precise location that results in a spike protein with extraordinary affinity/activity (furin cleavage site) with the human ACE2 receptor, making it extraordinarily contagious and infectious. 

Since 1992 there have been at least 11 separate experiments adding a special sequence to the same location. The end result has always been supercharged viruses.

And, in the entire class of coronaviruses that includes CoV-2, the CGG-CGG combination has never been found naturally. 

Although the double CGG is suppressed naturally, the opposite is true in laboratory work. The insertion sequence of choice is the double CGG. That’s because it is readily available and convenient, and scientists have a great deal of experience inserting it.